Use of Cary 5000 UV-Vis-NIR Spectrophotometer 175-3300 nm

Username: Cary

Password: cary (all lowercase)

Config:
波长范围：175～3300 nm；

波长准确度：±0.08 nm（紫外可见），±0.4 nm（近红外）；

分辨率：≤0.048 nm（紫外可见），≤0.2 nm（近红外）；

杂散光：0.00007%T；带宽：0.01～5 nm可调；

光度范围：8 Abs

choose scan program

wait for the machine come into “online” status
Setup: two things to be Setup
- cary option-mode/scan rate/wave length (simple read?)
- baseline: pick baseline correction, then ok
Go to the left bar-Calibration with the machine
set the zero (press zero button) to do Calibration.

blank sample means you calibrate with the solvent you will used in the procedures
when blank sample data comes out, press baseline button at the left bar

now the baseline of absorbance is set.
After baseline set, message saying “block the sample beam and press ok to collect the 0 %T baseline scan”
then block beam (use black plate to block the beam from the fright)

then the baseline is established from two aspects: absorbance and transmissivity （透射率）
then remove the black card, load sample to measure. (the triangle side should be the left)

press “start” button to activate
remove the black card—-then add samples—–start
After reading, click “ok”.

click “finish” to do save as—in your folder
finished—-save methods (choose right format to save—CSV format)

tips

Before and after, wash your cuvettes.
When running, you can press the scale button/up and down to choose the right frame.
when you wanna know the peak coordinates, press the peak label button to know.
if your samples are concentrated, put sample first and then add solvent…
add axis scale to zoom in what your sample is

Two forms of cuvetts.

Glass one clear sides/opaque sides.